CHOP CHOP v2 now available
CHOPCHOP v2: a web tool for the next generation of CRISPR genome engineering. Labun et al. Nucleic Acids Res 2016
CRISPR has brought a revolution to the field of genome engineering. The technology works as a molecular scissor, cutting the genomic DNA in a location specified by a complementary RNA guide. While CRISPR is in principle easy there exists many practical hurdles and challenges related to experimental design. An ideal experiment maximizes the cutting efficiency while at the same time prevent cutting at aberrant loci, thereby optimizing both efficiency and specificity.
Our software & website helps optimize these criteria according to the latest research and allows the biologist to focus on lab work. A simple yet informative visualization guides the selection of a location to cut within the target gene and allows the design of one or multiple guide RNAs. In the newest release we now support a large number of novel genomes and also adds support for alternative optimization schemes and the use of Cas9 homologs like Cpf1.
CHOPCHOP has since its inception been one of the most popular tools in CRISPR design and with this new release remains at the cutting edge of development.